Статья
МОЛЕКУЛЯРНЫЕ МАРКЕРЫ СД2 ПРИ ОЖИРЕНИИ: ЭКСПРЕССИОННЫЙ ПРОФИЛЬ ГЕНОВ, КЛЮЧЕВЫХ МОЛЕКУЛ ГЕНОВ ВОСПАЛИТЕЛЬНОГО ОТВЕТА В МОНОНУКЛЕАРНЫХ КЛЕТКАХ ПЕРИФЕРИЧЕСКОЙ КРОВИ
Type 2 diabetes mellitus (T2DM) is a metabolic multifactorial, genetically determined, life-threatening disease. The development of complications is associated with a chronic immuno-inflammatory process and with the formation of immune complexes. The aim of the study was to analyze the expression profile of a complex of functionally interconnected genes (cytokines, chemokines, and other key molecules of the inflammatory response) in peripheral blood mononuclear cells in patients with T2DM (N = 10) and in the control group (N = 10). Patients and controls were matched by gender, age, and belonged to the Tatars ethnic group. The expression profile of 84 immune response genes was studied by OT-PCR on a BioRad CFX96TM instrument using the Human Cytokines & Chemokines PCR Array RT2 Profiler PCR Arrays kit (Qiagen). Significant changes in expression (p
