PT - JOURNAL ARTICLE
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DP - 2020-09-04
4100 - 10.1016/j.toxlet.2019.03.008
SO - https://repo.bashgmu.ru/publication/91
AB - Arsenictrioxide(ATO)hasbeenrecommendedasthefirst-lineagentforthetreatmentofacutepromyelocytic leukaemia(APL),duetoitssubstantialanticancereffect.NumerousclinicalreportshaveindicatedthatATOisa developmentaltoxicantwhichcanresultinbirthdefectsofhumanbeings.Butwhetherarsenictrioxidecanlead to human cardiac developmental toxicity remains largely unknown. So the present study aims to explore the influenceandmechanismsofATOonhumancardiacdevelopmentbyusingavitrocardiacdifferentiationmodel ofhumaninducedpluripotentstemcells(hiPSCs).Herewefoundthatclinicallyachievableconcentrations(0.1, 0.5 and 1μM) of ATO resulted in a significant inhibition of proliferation during the whole process of cardiac differentiationofhiPSCs.Meanwhile,TUNELassayrevealedthatATOcouldcausecellapoptosisduringcardiac differentiationinaconcentration-dependentmanner.Consistently,wefoundthatATOreducedtheexpressions ofmesodermmarkersBrachyuryandEOMES,cardiacprogenitorcellmarkersGATA-4,MESP-1andTBX-5,and cardiacspecificmarkerα-actininindifferentiatedhiPSCs.Furthermore,ATOtreatmenthadcausedDNAdamage which was shown in the upregulation of γH2AX, a sensitive marker for DNA double-strand breaks. Taken together, ATO blocked cardiomyocyte differentiation, induced apoptosis and cell growth arrest during cardiac differentiationofhiPSCs,whichmightbeassociatedwithDNAdamage.