17 Valentin V. Kataev Valentina N. Perfilova Igor I. Prokofiev Alla A. Nesterova Yulia K. Isaeva Margarita V. Kustova Ivan N. Turenkov Anatoly K. Brel Kataev, V.A. chronic heart failure mitochondrial dysfunction oxidative stress hydroxybenzoic acid derivatives morphometric parameters of cardiomyocytes compound F-26 mildronate Scopus ВАК Белый список 2025 2026-05-18 DOI 10.18413/rrpharmacology.11.811 RESEARCH RESULTS IN PHARMACOLOGY Introduction: Chronic heart failure (CHF) is a leading public health problem. Its most common manifestation is decreased cardiac inotropic reserve. Mitochondrial dysfunction and oxidative stress contribute to the progression of CHF. Hydroxybenzoic acid derivatives limit mitochondrial dysfunction and oxidative stress, thereby reducing their damaging effects on the heart. Materials and Methods: Chronic heart failure (CHF) was modeled by intraperitoneal administration of L-isoproterenol to male Wistar rats at a dose of 2.5 mg twice daily for 21 days. Four groups of 14 animals each were formed: intact animals, a control group with CHF that received saline solution, and experimental animals with CHF that received compound F-26 at a dose of 7.9 mg/kg and mildronate –50 mg/kg intraperitoneally once daily throughout the CHF simulation period. Cardiac inotropic function was assessed using functional tests: volume loading, adrenergic receptor activation, and occlusion of the ascending aorta. Mitochondrial oxygen uptake was determined using polarography. Myocardial morphometric analysis was performed using micrographs. Results and Discussion: Compound F-26 has a positive effect on cardiac inotropic reserves, as evidenced by increases in myocardial contraction and relaxation rates, left ventricular pressure, and maximum structural activity intensity (MSI) during functional tests. Compound F-26 improves mitochondrial respiration, reduces MDA concentration, increases SOD activity, and has a positive effect on the cross-sectional area, thickness, and volume fraction of cardiomyocytes and interstitium. Conclusion: Compound F-26 improves cardiac inotropic function, mitochondrial function, the antioxidant system of cardiomyocytes, and morphometric parameters. https://repo.bashgmu.ru/publication/5346 https://repo.bashgmu.ru/files/5539