Publication date: 2019

DOI: 10.1002/eji.201970300

Prostate cancer (PC) is the second most frequently diagnosed malignant tumor and the sixth leading cause of death for cancer in the male population. Every year, over 899,000 new cases are diagnosed in the world, which is 14% of all cases of cancer. The recruitment of migratory hematopoietic cells, including tumor-associated macrophages, to the supporting stroma is essential in the progression and metastasis of tumors. One of the main macrophages function is phagocytosis of apoptotic cells, that can induce secretion of key factors implicated in tumor progression. Besides the lack of specificity and sensitivity of modern diagnostic methods leads to the search for new factors that make it possible to predict the development of metastasis in a PC patients. To explore the role of phagocytic macrophages in prostate cancer lymph node metastasis immunohistochemical (IHC) analysis was performed. IHC staining of the 37 human prostate cancer paraffin embedded and cutted tissue samples (13 with and 24 without lymph node metastasis) was done using mouse antibody to CD68 (1:50; Zytomed Systems). Large phagocytic "foamy" and small non-phagocytic cells were counted in 10 fields of each tissue samples using Leica DMRE light microscope with x100 magnification. GraphPad Prism 7.03 program was used for Mann Whitney U-test.

Издатель: Wiley Interscience

Тип: Article